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  • LAB WORK: Observation of the phases of mitosis
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      LAB WORK: Observation of the phases of mitosis
      CELLS AND THE CELL CYCLE
      Without background sound

      What can you see in the phases of mitosis?

      As you know, mitosis is the process of the division of the nucleus of a cell. It ensures that equal parts of genetic material are given to each daughter cell that originates after cytokinesis.

      In plants, mitosis occurs mainly in meristems, which are the tissues that allow the organism to grow. They are found, among other places, in the tips of the stem and the roots.

      In the following video, you will see the process necessary to observe meristematic cells in mitosis through a microscope.

      /useruploads/ctx/a/469637430/r/s/140198854/ESPM000379513.mp4?idcurso=4518326
       ESPM

       

      LAB WORK: Observation of the phases of mitosis
      CELLS AND THE CELL CYCLE
      Without background sound

      Understanding the process (I)

      Why do you think the sample must be very small and flattened? (Choose more than one reason.)

      • To allow light to pass through the sample.

      • Correct answer
        Wrong answer

      • To allow only one layer of cells to better observe them.

      • Correct answer
        Wrong answer

      • To remove excess stain.

      • Correct answer
        Wrong answer

      • So it fits under the lens.

      • Correct answer
        Wrong answer

        attempts
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      LAB WORK: Observation of the phases of mitosis
      CELLS AND THE CELL CYCLE
      Without background sound

      Understanding the process (II)

      Why do you think it's easier to observe the phases of mitosis in cells from the tips of the roots than in other parts of the plant? (Choose more than one reason.)

      • It's easier to cut a sample there.

      • Correct answer
        Wrong answer

      • The meristematic tissue is growing there.

      • Correct answer
        Wrong answer

      • The cells are continuously in prophase.

      • Correct answer
        Wrong answer

      • The cells there are dividing continuously.

      • Correct answer
        Wrong answer

        attempts
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      LAB WORK: Observation of the phases of mitosis
      CELLS AND THE CELL CYCLE
      Without background sound

      Understanding the process (III)

      Why is the sample stained with methelyne blue stain?

      • Without it the cells would die.

      • Correct answer
        Wrong answer

      • It makes photos nicer.

      • Correct answer
        Wrong answer

      • Without it the DNA would not be visible.

      • Correct answer
        Wrong answer

      • It keeps the DNA from separating.

      • Correct answer
        Wrong answer

      • It flattens the cells.

      • Correct answer
        Wrong answer

        attempts
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      LAB WORK: Observation of the phases of mitosis
      CELLS AND THE CELL CYCLE
      Without background sound

      Understanding the lab work

      Mark each statement true or false.

      • It's easiest to observe chromosomes in the metaphase because it is when they are completely condensed, each one with two chromatids.
      • Correct answer
        Wrong answer
      • In the lab, other structures such as centrioles and spindles can be seen, besides the chromosomes.
      • Correct answer
        Wrong answer
      • The formation of the phragmoplast occurs during cytokinesis, at the end of the telophase.
      • Correct answer
        Wrong answer
      • You can cut any part of the onion root to observe mitosis easily.
      • Correct answer
        Wrong answer
      • First, use the lowest magnification to locate the cells.
      • Correct answer
        Wrong answer
      • Nucleoli can be observed at the beginning of the prophase and the end of telophase.
      • Correct answer
        Wrong answer

        attempts
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      LAB WORK: Observation of the phases of mitosis
      CELLS AND THE CELL CYCLE
      Without background sound

      Which phase is it?

      Connect the number to the correct phase.

      Observe the image of the meristematic cells from the onion root in different phases of the cell cycle.

      • 1

      • 2

      • 3

      • Prophase

      • Metaphase

      • Interphase

        attempts
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      LAB WORK: Observation of the phases of mitosis
      CELLS AND THE CELL CYCLE
      Without background sound

      Lab test

      Choose the correct answers.

      • Which phase is this cell in?

      • Correct answer
        Wrong answer

      • Which structure is circled in the image?

      • Correct answer
        Wrong answer
      • Why do the roots of the cebolla need to be placed in water for a few days before the lab?
      • Correct answer
        Wrong answer
      • Which phase is best to observe the spindle?
      • Correct answer
        Wrong answer

        attempts
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      LAB WORK: Observation of the phases of mitosis
      CELLS AND THE CELL CYCLE
      Without background sound

      Think about it . . .

      Meristematic tissue allows an organism to grow. The tiny cells undergoing mitosis are the reason plants can grow, many of them providing food which helps us to grow and stay healthy. 

      When you were small, you probably planted a bean in some cotton wool to observe the way it sprouted and grew. Think about everything that you know now about what was happening on the inside of that little plant. The cells are going through an amazing process of continuous division. The genetic material within the nucleus is being divided precisely to create new cells. Without the development of microscopes this tiny world would have stayed a mystery!


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